Author  
Place of duty  
Title   대장암조직에서 염색체 열성대립유전자 결손 ( Recessive Allelic Loss in Colorectal Cancer Tissue )
Publicationinfo   1993 Jan; 025(06): 848-855.
Key_word   Allelic deletion, RFLP, Suppressor gene
Full-Text  
Abstract   Two types of genetic alterations have been reported in colorectal tumors. The first type involves point mutations in ras proto-oncogenes. The second type of alterations involves deletion of specific chromosomal regions. Deletions can be detected by restriction fragment lenght polymorphism analyisis of tumor DNA. The deleted sequences have been hypothesized to include tumor suppressor gene. The inactivation of tumor-suppressor gene by deletian of suspected locus lead to neoplatic growth. To investigate the relation between ras point mutation and allelic deietion of chromosome 17p and 18q, both p-PCR and RFLP analysis using VNTR marker and synthetic oligonulectide probe tailing with digoxigenin l1-dUTP were done in 15 normal mucosa and 22 colorectal cancer mucosa. The obtained results were as follows; 1) Incidence of ras point mutations was 80%(4/5) in Dukes B, 76.4%(l3/17) in Dukes' C. There was increasing tendency of mutation along with stage progression, but no statistical significance was noted. 2) Analysis of incidence of mutations over 2 points in the same DNA sequence between well differentiated and moderately differentiated cancer did not show any statistical significance. 3) Incidence of allelic deletion of l7p pYNH37 were 60%(3/5) in Dukes B, 46.7%(11/17) in Dukes C. There was no correlation of incidences of 18q 05-4 in colorectal cancer. But inci- dence of 17p allelic deletion was higher than 18q allelic deletion in Dukes C colorectal cancer. The allelic deletions of p YNZ22 were noted in 6 cases of Dukes C colorectal cancer but not in dukes' B colorectal cancer. 4) There was no statistical difference between incidence of ras point mutation and allelic deletion in colorectal cancer. These results showed there was no statistical difference in incidence of ras point mutation & 18q allelic deletion in colorectal cancer. 17p allelic deletion occurred more frequently in advanced colorectal cancer. Genetic alteration did not have any relation with cellular differentiation or tumor location in the biologic behavior of the colorectal cancer.
저 자   조원일(Won Il Cho),유승진(Seung Jin Yoo),장석균(Suk Kyun Chang),김승남(Seung Nam Kim),송영택(Young Tack Song),이재학(Jai Hak Lee),주상용(Sang Yong Choo)